The peptide nucleic acids (PNAs): introduction to a new class of probes for chromosomal investigation
Identifieur interne : 003110 ( Main/Exploration ); précédent : 003109; suivant : 003111The peptide nucleic acids (PNAs): introduction to a new class of probes for chromosomal investigation
Auteurs : Franck Pellestor [France] ; Petra Paulasova [République tchèque]Source :
- Chromosoma [ 0009-5915 ] ; 2004-06-01.
Abstract
Abstract: Peptide nucleic acids (PNAs) are synthetic DNA mimics in which the sugar phosphate backbone is replaced by repeating N-(2-aminoethyl) glycine units linked by an amine bond and to which the nucleobases are fixed. Peptide nucleic acids hybridize with complementary nucleic acids with remarkably high affinity and specificity, essentially because of their uncharged and flexible polyamide backbone. The unique physicochemical properties of PNAs have led to the development of a large variety of biological research assays, and, over the last few years, PNAs have proved their powerful usefulness in genetic and cytogenetic diagnostic procedures. Several sensitive and robust PNA-dependent methods have been designed for modulating polymerase chain reactions, detecting genomic mutation or capturing nucleic acids. The more recent applications of PNA involve their use as molecular hybridization probes. Thus, the in situ detection of several human chromosomes has been reported in various types of tissues.
Url:
DOI: 10.1007/s00412-004-0282-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001159
- to stream Istex, to step Curation: 001159
- to stream Istex, to step Checkpoint: 000B68
- to stream Main, to step Merge: 003142
- to stream Main, to step Curation: 003110
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The peptide nucleic acids (PNAs): introduction to a new class of probes for chromosomal investigation</title><author><name sortKey="Pellestor, Franck" sort="Pellestor, Franck" uniqKey="Pellestor F" first="Franck" last="Pellestor">Franck Pellestor</name></author><author><name sortKey="Paulasova, Petra" sort="Paulasova, Petra" uniqKey="Paulasova P" first="Petra" last="Paulasova">Petra Paulasova</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:322070EE8CE4556806E535B5E3CC2C93DDAD425F</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1007/s00412-004-0282-8</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-FKKJV6M5-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001159</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001159</idno><idno type="wicri:Area/Istex/Curation">001159</idno><idno type="wicri:Area/Istex/Checkpoint">000B68</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B68</idno><idno type="wicri:doubleKey">0009-5915:2004:Pellestor F:the:peptide:nucleic</idno><idno type="wicri:Area/Main/Merge">003142</idno><idno type="wicri:Area/Main/Curation">003110</idno><idno type="wicri:Area/Main/Exploration">003110</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The peptide nucleic acids (PNAs): introduction to a new class of probes for chromosomal investigation</title><author><name sortKey="Pellestor, Franck" sort="Pellestor, Franck" uniqKey="Pellestor F" first="Franck" last="Pellestor">Franck Pellestor</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CNRS UPR 1142, Institute of Human Genetics, 141 rue de la Cardonille, 34396 Montpellier Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author><author><name sortKey="Paulasova, Petra" sort="Paulasova, Petra" uniqKey="Paulasova P" first="Petra" last="Paulasova">Petra Paulasova</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Centre of Assisted Reproduction and Reproductive Genetics, Institute of Biology and Medical Genetics, Motol Hospital, V uvalu 84, 150 06 Prague 5</wicri:regionArea><wicri:noRegion>150 06 Prague 5</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Chromosoma</title><title level="j" type="abbrev">Chromosoma</title><idno type="ISSN">0009-5915</idno><idno type="eISSN">1432-0886</idno><imprint><publisher>Springer-Verlag; http://www.springer.de</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2004-06-01">2004-06-01</date><biblScope unit="volume">112</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="375">375</biblScope><biblScope unit="page" to="380">380</biblScope></imprint><idno type="ISSN">0009-5915</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0009-5915</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Peptide nucleic acids (PNAs) are synthetic DNA mimics in which the sugar phosphate backbone is replaced by repeating N-(2-aminoethyl) glycine units linked by an amine bond and to which the nucleobases are fixed. Peptide nucleic acids hybridize with complementary nucleic acids with remarkably high affinity and specificity, essentially because of their uncharged and flexible polyamide backbone. The unique physicochemical properties of PNAs have led to the development of a large variety of biological research assays, and, over the last few years, PNAs have proved their powerful usefulness in genetic and cytogenetic diagnostic procedures. Several sensitive and robust PNA-dependent methods have been designed for modulating polymerase chain reactions, detecting genomic mutation or capturing nucleic acids. The more recent applications of PNA involve their use as molecular hybridization probes. Thus, the in situ detection of several human chromosomes has been reported in various types of tissues.</div></front></TEI><affiliations><list><country><li>France</li><li>République tchèque</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Pellestor, Franck" sort="Pellestor, Franck" uniqKey="Pellestor F" first="Franck" last="Pellestor">Franck Pellestor</name></region><name sortKey="Pellestor, Franck" sort="Pellestor, Franck" uniqKey="Pellestor F" first="Franck" last="Pellestor">Franck Pellestor</name></country><country name="République tchèque"><noRegion><name sortKey="Paulasova, Petra" sort="Paulasova, Petra" uniqKey="Paulasova P" first="Petra" last="Paulasova">Petra Paulasova</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003110 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003110 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:322070EE8CE4556806E535B5E3CC2C93DDAD425F
|texte= The peptide nucleic acids (PNAs): introduction to a new class of probes for chromosomal investigation
}}
| This area was generated with Dilib version V0.6.33. |  |